| OBJECTIVE |
| EDUCATION |
| RESEARCH AND PROFESSIONAL EXPERIENCE |
| AWARDS & HONORS |
| Seeking a Scientist/Crystallographer position in BioPharma industry studying structures of signaling molecules and drug targets, focusing on protein-protein or protein-drug interactions relevant to drug discovery and development. A position that utilizes current knowledge of protein biochemistry and X-ray crystallography and provides opportunities to enhance skills with state-of-the-art technology in a stimulating work environment is preferred. |
| Boston University School of Medicine University of Massachusetts Medical School University of Rochester |
| Fellow (2001-present) Wyeth (formerly Genetics Institute), Cambridge, MA Crystallography Group; Dept. of Chemical & Screening Sciences; Crystallographic and biochemical studies of ligand-receptor complexes. Postdoctoral Research Associate (1997-2001) University of Massachusetts Medical School, Worcester, MA Laboratory of David Lambright, PhD; Program in Molecular Medicine; Crystallographic and biochemical studies of proteins that regulate membrane trafficking. Graduate Student/Research Assistant (1992-1997) Boston University School of Medicine, Boston, MA Laboratory of Norman Boyd, PhD; Dept. of Pharmacology & Experimental Therapeutics; Biochemical studies of the substance P (NK-1) receptor using photoreactive ligands and cross-linking reagents. Graduate Student (1990-1991) University of Massachusetts Medical School, Worcester, MA Research Assistant II (1989-1990) Children's Hospital/Harvard Medical School, Boston, MA Laboratory of Sotirios Karathanasis, PhD; Dept. of Cardiology; Studies of the transcriptional activity of the gene encoding apolipoprotein A-1, a protein involved in cholesterol transport. Research Assistant I (1987-1989) Harvard School of Public Health, Boston, MA Laboratory of Lester Kobzik, MD; Physiology Program; Studies of oxidant responses to pathogens in lung macrophages, and cytokine-dependent effects on phagocytic and transcriptional activities. Unit Coordinator (1983-1997) Beth Israel Hospital - Emergency Unit, Boston, MA |
| Wyeth Discovery Annual Meeting - Outstanding Poster Presentation Wyeth - Chemical & Screening Sciences - Distinguished Publication Award NIH Postdoctoral Fellowship H.I. Russek Student Achievement Award, honorable mention SmithKline Beecham Graduate Student Award |
| X-ray crystallography: Crystal growth and screening (manual and robotic), data collection (home source R-axis detectors, ALS 5.0.1 and NSLS X4A/X12C), data processing (Denzo, Mosflm), phase determination by molecular replacement (AMoRe, CNS) and multiwavelength anomalous dispersion (Shelx, Sharp), model building (Quanta, O), model refinement (CNS, Refmac/CCP4), molecular graphics (Ribbons, PyMOL, GRASP, MolScript/Raster3D, Midas). Protein biochemistry & molecular biology: Design and construction of DNA expression vectors, PCR-based site-directed mutagenesis, high level expression of protein in bacteria, protein refolding, protein purification (affinity, ion-exchange, and size exclusion chromatography). Bioanalytical electrophoretic techniques, SDS-PAGE and immunoblot analysis. Analysis of protein-protein interactions by surface plasmon resonance assay (Biacore), size-exclusion chromatography, analytical ultracentrifugation, gel shift assay, and co-precipitation. Receptor biochemistry: Analysis of peptide binding site using radiolabeled, photoreactive ligands, analysis of complex formation by chemical cross-linking, peptide mapping by enzymatic and chemical cleavage, analysis of ligand affinity by equilibrium binding/rapid filtration and changes in intrinsic tryptophan fluorescence, and kinetics of receptor endocytosis. Computer experience: MacOS, Windows, and UNIX systems; molecular graphics on SGI system. |
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| John J. Dumas |
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| 49 Hamlet Street Arlington, MA 02474 |
| Boston, MA Worcester, MA Rochester, NY |
| Ph.D. M.S. B.A. |
| Pharmacology Medical Science Biology/Psychology |
| 1998 1994 1986 |
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| SKILLS & TECHNIQUES |
| 2004 2003 1998-2000 1997 1995 |
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